Research article
Aquaria flushing technique of rosella extract (Hibiscus sabdariffa Linn) to determine the value of SGOT and SGPT in milkfish (Chanos chanos) induced by heavy metals (Hg)

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The purpose of this study was to determine the value of SGOT and SGPT in milkfish induced by heavy metals (Hg) by aquaria flushing technique with Rosella (Hibiscus Sabdariffa Linn) extract. The samples used were 30 male adult milkfish divided into 5 groups. The treatment group was induced with a toxic dose of heavy metal mercury (Hg), which was dissolved in a volume of m3 of water. Blood collection from the heart vein of milkfish as much as 1-2 ml for SGPT and SGOT examination was carried out using the Berthelot method. The liver, gills and brain of milkfish were then examined, 300 grams were centrifuged until smooth and then dissolved in methanol to obtain concentrations of 25ppm, 50ppm, 100ppm, 200ppm, 300ppm, 400ppm, 500ppm, 600ppm, 700ppm, 800ppm, 9 00ppm. and 1000ppm then analyzed by ANOVA. Based on the research results of black roselle flower extract therapy with aquaria flushing system on male milkfish, the average value of SGOT and SGPT showed a very significant decrease in groups P1, P2, P3, and P4, against the P0 group or control group (P<0, 01 ).


Rosella extract
Mercury (Hg)
Flushing Technique

Funding Information

Lembaga Penelitian dan Pengabdian Masyarakat (LPPM) Universitas Wijaya Kusuma Surabaya


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No ethical approval required for this article.

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No conflict of interest has been declared by the authors.

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Cite this article as:

Hermawan, I.P., Astuti Nussa, O.R., & Kurnianto, A., 2022. Aquaria flushing technique of rosella extract (Hibiscus sabdariffa Linn) to determine the value of SGOT and SGPT in milkfish (Chanos chanos) induced by heavy metals (Hg). Agrikan: Jurnal Agribisnis Perikanan 15(2): 350-354.
  • Submitted
    24 August 2022
  • Revised
    6 June 2023
  • Accepted
    Not available
  • Published
    4 October 2022
  • Issue date
    30 October 2022


Intan  Permatasari Hermawan

IntanPermatasariHermawan, Universitas Wijaya Kusuma Surabaya, Indonesia., scholarGoogle Scholar Profile

Olan  Rahayu Puji Astuti Nussa

OlanRahayu PujiAstuti Nussa, Universitas Wijaya Kusuma Surabaya, Indonesia.

Ady  Kurnianto

AdyKurnianto, Universitas Wijaya Kusuma Surabaya, Indonesia.

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