Vaname shrimp is one of the fishery products that are expected to produce foreign exchange for the country besides tiger shrimp.  One factor that can affect the growth and the production of vaname shrimp culture is dense stocking. The research was conducted in October-December 2016 at the Balai Benih Ikan Pantai (BBIP) Ghonebalano, aims to determine the effect of different stocking density on the growth and survival of vaname shrimp.  The research was using Completely Randomized Design with three treatment levels of density, i.e. treatment A = 10 individuals per container, treatment B = 15 individuals per container and treatment C = 20 individuals per container and three replications.  The results showed that the highest growth was obtained in treatment A which was 1,563 g/individuals, compared with treatment B (1.189 g/individuals) and C treatment (0.834 g/individuals).  Survival rate in treatment A is 80.00%, B treatment equal to 75,56% and treatment C equal to 70,00%.  Analysis of variance at 95% confidence level (α0.05) showed that the different density had a significant effect on growth, but had no significant effect on survival rate of the vaname shrimp larvae.

The study aims to determine the optimum temperature, optimum density and optimum temperature and density interactions in milkfish (Chanos chanos) transportation. This research was conducted in 2016, in Kendari City, Southeast Sulawesi Province. This study uses a factorial design, consisting of Temperature and Density Factors. The design of the combination treatment of Temperature (A) namely Temperature 15 oC, A2 Temperature 18 oC, A3 Temperature 21 oC. For Density Factor (B), B1 Density of 600 individuals / container, B2 Density of 800 individuals / container, B3 Density of 1,000 individuals / container. The results of analysis of variance at 95% and 99% confidence level, the treatment in this study had no significant effect, where the F count is smaller than the F table. This shows that the combination of temperature and density tested in this study can be used for energy transportation activities for 14 hours. Based on the percentage of graduation of nener life tested in the transportation, starting from the biggest graduation to the smallest life pass, is a combination of 15 oC temperature treatment and a density of 1000 individuals 95.57%, a combination of 18 oC temperature treatment and a density of 800 individuals 95.04 %, a combination of temperature treatment of 21 oC and density of 1000 individuals 91.70%, combination of temperature treatment of 15 oC and density of 800 individuals 90.04%, combination of temperature treatment of 15 oC and density of 600 individuals 89.89%, combination of temperature treatment of 21 oC and a density of 800 individuals 88.67%, a combination of temperature treatment 18 oC and a density of 1000 individuals 88.07%, a combination of temperature treatment 21 oC and a density of 600 individuals 84.33% and a combination of temperature treatment 18 oC and density of 600 individuals 73.28%.

The study aimed to determine the growth and survival of Litopenaeus vannamei shrimp with the application of probiotics to different stocking densities.  The study was conducted from May to June 2018 at the Balai Benih Ikan Pantai (BBIP) Ghonebalano, Duruka District, Muna Regency, using a Completely Randomized Design (CRD) with three levels of solid stocking treatment namely treatment A is 18 individu per container, treatment B is 24  individu per container and treatment C is 30  individu per container with three replications.  Data analysis using Variance Analysis (ANOVA) at the confidence level of 95% (α 0.05).  The results showed that the highest daily growth was obtained at 24 individual densities of 5.88% per day, compared to 18 individual densities of 5.86% per day and 30 individual densities of 5.74% per day.  The highest absolute growth is obtained at 24 individual density of 2.43 g per individu, then 18 individual density of 2.15 g per individual, and the lowest at 30 individual density of 2.02 g per individual.  Survival at a density of 18 individual and a density of 24 individual at 88.89%, while a 30-ind density of 86.67%.  Analysis of variance (α 0.05) showed that the application of probiotics to different stocking densities had no significant effect on the daily growth rate of vannamei shrimp (p<0.939), absolute growth of vannamei shrimp (p<0.080), and survival of vannamei shrimp (p<0.744).

One of the factors that affect the growth of tiger shrimp is the quality of feed. Nutrient content in feed such as protein, carbohydrate and fat, generally used as a parameter of feed quality. Feed consumed by shrimp is not all digested, one of which depends on the enzymatic ability of the shrimp digestive tract. The use of probiotic bacteria in addition to having the ability to reduce colonies, inhibit the growth of pathogenic bacteria, and neutralize water quality, probiotic bacteria can help digestibility of the intestine. The aim of this research is to know the growth and survival of post larvae of shrimp (Penaeus monodon Fabr.) Through the provision of probiotics with different doses. The research was conducted at Balai Fish Fishing Hall of Muna Regency, Southeast Sulawesi, Indonesia. Post larva shrimp tiger (PL-20) of 120 tails, maintained for 28 days. The tested treatment was probiotic dose 5 ml (treatment A), dose 10 ml treatment B), dose 15 ml (treatment C), and without probiotics (control). Probiotics are given every 7 days on water maintenance media using a pipette (0.5-20 ml scale). Weighing results showed relatively high post-larva shrimp larvae growth of 400.00% (treatment B); 291.67% (treatment C); of 233.33% (treatment A) and equal to 58.33% (Control). Variation analysis result showed that giving of probiotic with different dose gave real effect (P<0,01) to relative growth of postlarva prawn weights (P. monodon Fabr.). Provision of probiotics has no significant effect on postlarva tiger prawn survival rate (P. monodon Fabr.). The survival rate of post larvae of tiger shrimp during the study was in the range 66.67-83.33%.

The plant species of miana (Coleus scutellariodes) has been historically recognised for its antiviral properties.  However, there is currently no scientific evidence supporting the efficacy of miana leaf extract in preventing White Spot Syndrome Virus (WSSV) in tiger prawns (Penaeus monodon) when administered via feed. This study aimed to determine the impact of including miana leaf extract in the diet on the  survival percentage of tiger prawns that have been infected with the WSSV. The study was carried out between June and August 2023 in the Laboratory of Parasite and Fish Disease and the Hatchery Laboratory of Hasanuddin University. This study employed a completely randomized experimental design consisting of five distinct treatments, each with three replicates. The experimental treatments included the control group (treatment A), where food was provided without the inclusion of miana leaf extract. Furthermore, there were four treatment groups (treatments B, C, D and E) where feed was supplemented with miana leaf extract at concentrations of 125, 250, 500, and 750 µg mg^-1 feed, respectively. Rearing was performed in two phases: preinfection (25 days) and post- infection (7 days). The statistical results of the statistical on pre-WSSV infection indicated that the addition of miana leaf extract to the food resulted in survival that was not significantly different (p>0.05). The results were different post-infection, significantly (p<0.05) the shrimp fed with the addition of miana leaf extract were higher than those without the extract. Furthermore, there was a significant difference in shrimp survival between pre- and post-WSSV infection. WSSV mitigation through immunostimulants using miana leaf extract, which is inexpensive and environmentally friendly, has the potential to minimize the impact of WSSV infection.